[Spm] RE: Spm Digest, Vol 57, Issue 2: Image comparison and subtraction (Iain McNab)

[Anders Kühle]

Hello Iain,

In the software package SPIP(TM) (www.imagemet.com) you can align (shift) images automatically with sub-pixel accuracy and perform typical mathematical operations such as subtraction. You can even align a whole bunch of images to the same reference which we often use for creation of movies from a time series of images, thus keeping the background steady. The Sm4 format is directly supported in the latest version of the software. Cropping can be scripted but you will have to estimate/define the overlap region more or less manually.

You can see an example here: http://www.imagemet.com/index.php?main=products&sub=examples&id=68

Best regards
Anders 
-----------------
Anders Kühle, Technical Product Manager
Image Metrology
Phone +45 469 234 00
Fax +45 469 234 01
ak at imagemet.com

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Subject: Spm Digest, Vol 57, Issue 2

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Today's Topics:

   1. Postdoc in single molecule conductivity  (Eric U Borguet)
   2. image comparison and subtraction  (Iain McNab)


----------------------------------------------------------------------

Message: 1
Date: Tue, 31 Mar 2009 21:49:17 -0400 (EDT)
From: Eric U Borguet <eborguet at temple.edu>
Subject: [Spm] Postdoc in single molecule conductivity
To: spm at spmlist.di.com
Message-ID: <Pine.LNX.4.64.0903312144300.22172 at astro.ocis.temple.edu>
Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed

A postdoctoral research position in single molecule conductivity and scanning probe microscopy of molecular wires is available. Candidate must have experience with Atomic Force Microscopy (AFM) and Scanning Tunneling Microscopy (STM).  Candidates should have experience obtaining atomic and molecular resolution with STM and AFM in ambient and under fluid. 
Candidate should have expertise in electrochemistry, preparation of single crystal electrodes, and growth of self-assembled monolayers.

Focus of project is single molecule conductivity of peptide nucleic acid based structure, the effect of molecule electrode linker groups, and redox controlled conductivity, in collaboration with a team of experimentalists and theorists at the Carnegie Mellon University, the University of Pittsburgh and Duke University.

A strong record of publication in peer-reviewed literature attesting to
these capabilities is required.   Additional responsibilities include
training and supervision of students, and preparation of reports and manuscripts for publication.  Ph.D. in Chemistry or related discipline, awarded in the last last five years, is necessary. A background in physical chemistry or related discipline is desirable.

Temple University is an Equal Opportunity/Affirmative Action Employer, and specifically invites and encourages applications from women and minorities (AA, EOE, m/f/d/v.).

Applications should be addressed to:

Dr. Eric BORGUET
Department of Chemistry
201 Beury Hall
Temple University
1901 N. 13th Street
Philadelphia, PA 19122
USA

eborguet at temple.edu
http://www.temple.edu/borguet/






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Message: 2
Date: Tue, 31 Mar 2009 19:31:59 -0400 (EDT)
From: Iain McNab <imcnab at chem.utoronto.ca>
Subject: [Spm] image comparison and subtraction
To: spm at spmlist.di.com
Message-ID: <Pine.LNX.4.64.0903311931160.14489 at alchemy>
Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed

Dear All,

Could someone please recommend to me the best (and simplest) way of doing the
following:

(1) Comparing two images (that have drift between them) to find the best possible overlap (I'm guessing a correlation program of some sort).
(2) Then trimming both images so they show the same area.
(3) Subtracting the resulting images from one another.

I know I can do this "visually" in photoshop etc., but I'm hoping there is a more precise solution on offer. At present I have the images as both bitmaps, and also in RHK's native format, *.SM4.

All help will be most gratefully received, thank you for your time.

Best regards.... Iain McNab

URL:	http://www.utoronto.ca/jpolanyi
URL:    http://www.phys.ncl.ac.uk/research/atomic/irm/mcnabgroup.htm
**********************************************************************
Iain R. McNab
Department of Chemistry
University of Toronto
80 St. George Street
Toronto, Ontario, M5S 3H6
Canada

Tel. No.: (416) 978-3625
Fax No.:  (416) 978-7580
E-mail:   imcnab at chem.utoronto.ca
**********************************************************************


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End of Spm Digest, Vol 57, Issue 2
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