[Spm] Importing ASCII format image into Nanoscope software

[Narayanan Janakiraman]

Hello all,

In the mean time I have been still trying to read AFM images collected on a SIS machine into the Nanoscope software. I used SPIP program to convert the *.sis format image into an ASCII format image; this file data (height data in nanometers) could be read for example in softwares including WSxM, SPIP, gwiddion and also in Microcalc Origin. But the challenge to get this file imported into the Nanoscope software involves (1) creation of an equivalent header in the ASCII file, mimicking the one generated by the Nanoscope software; (2) converting the data from nanometers into LSB values and printing them in the ASCII file in the identical format as the nanoscope-generatd ASCII file containing data in RAW format.

I nearly achieved all the above, but the file generated by the import of such a modified ASCII file (using ASCII import command) in Nanoscope offline software is not displaying true height data. I foirst usd the conversion formulae for version 4.3 headers given in the link: http://www.physics.arizona.edu/~smanne/DI/software/v43header.html

This did not work as the above link does not consider the part "\@Z magnify: C [2:Z scale]" which does not seem to follow any specific trend or proportion to the height data in general. As for an arbitrary ASCII image, the values of  "\@Z magnify: C [2:Z scale]", "\@Sens. Zscan:" etc are not defined, I tried to assume arbitrary fixed values, and calculated zscale and height data in LSB as follows:

zscale = Round[(Max[Height Data in nm] - Min[Height Data in nm])/10] * 10;

Height Data in LSB[[i, j]]  =  Round[Height Data in nm[[i, j]] * 655.36 ] ;

The nanoscope realtime software selects appropriate values of above parameters for each image based on some logic. Any way, my purpose is only to read the height data truly into this software. 

Can any one suggest a simple procedure to import an ASCII image (with actual data such as height in nm arranged in say a 512*512 matrix) into nanoscope software, so I can proces it like a regular nanoscope file?

Thanks in advance.

Greetings,
Narayanan

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Dear Narayanan,

If you are using version 5.xx NanoScope software, you should be able to
 do an ASCII or TIFF file import into the NanoScope software in Offline
 via the Utility menu.  You just need to figure out how to convert your
 *.sis files to ASCII (or TIFF).

Sincerely,
Lisa Fukunaga
Applications Engineer
Veeco Instruments
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hello,

from your ask on SPM-digest,
have you test gwiddion software? In my opinion, this free and
open-source software very usefull.

sincerely,

Cyril


Herrier Cyril
phd position
university of rennes(35000), france
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