[Spm] different deflection sensitivity in two phases

[Sam Engler]

Hello Everyone,

I have a multiple composition lipid membrane sample, which phase-separated into 3 phases. During the force-displacement measurements, the slopes of the f-d curves within phase C are different than the slopes within phase A and B. I've tried different cantilevers and this is consistent. My questions are (1) why the deflection sensitivity of the cantilever is changing from one phase to another phase? (2) should I change to stiffer cantilevers?

Any comments will be appreciated. 

Thanks,
Sam,

Sam Engler, Ph.D


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