[Spm] adhesion of DNA/e-coli on the silanized silicon

[Poddar,Pankaj]

Hi, 

We are struggling with getting fluid cell images on the silanized (APTES
coated) silicon/mica substrate. AFM and ellipsometry results confirm a good
layer of APTES but when we put DNA or microbial cells (such as ecoli etc.)
by using standard protocols, we don't find DNA/cells on the substrate. We
tried changing the concentration of DNA/cells but it did not help.

 

We will appreciate if somebody could help us getting the DNA/cell images in
the fluid cell. We are using Multimode SPM with Nanoscope IV controller
with NPS probes from Veeco.

 

-Pankaj Poddar

 

========================

Dr. Pankaj Poddar

Physical & Materials Chemistry Division

National Chemical Laboratory

Dr. Homi Bhabha Road

Pune 411008

India

Tel.   : +91-20-2590-2580

Fax    : +91-20-2590-2636

E-mail : p.poddar at ncl.res.in

 <http://www.ncl-india.org> www.ncl-india.org

 

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