[Spm] blunt pyramidal tips

[Martin Stolz]

Dear Valeria,
 
Back in 1997, we wanted to perform indentation testing on cartilage 
using larger contact areas. Therefore, we ordered diamond-like carbon 
(DLC)-coated glass from Zeiss. The idea was to produce blunt AFM tips by 
scratching at high force over the DLC surface. I remember scratching 
half a day with a single Si-tip on the DLC coating, but the tip did not 
get significantly blunt.
 
Next, we glued borosilicate glass spheres onto tipless cantilevers. If 
you have a good micromanipulation stage (we are using Veeco’s Bioscope 
AFM) you can glue spheres of down to about 1 micron diameter. Another 
advantage is that you’re having defined tip geometry for calculation the 
stiffness. Meanwhile, you’ll find several protocols for gluing spheres 
on the internet. Also companies are now selling such spherical indenters…
 
I just thought while reading your email that you probably not want to 
repeat my mistakes.
 
Best Regards,
 
Martin
 
 
------------------------------------------------------------------
Dr. Martin Stolz
Maurice E. Mueller Institute for Structural Biology  
Biozentrum/University of Basel
Klingelbergstr. 70
4056 Basel
Switzerland
tel: 0041 61 2672095
fax: 0041 61 2672109
email: martin.stolz at unibas.ch
http://www.mih.unibas.ch <http://www.mih.unibas.ch/>
Swiss NCCR on NANOSCIENCE  http://www.nccr-nano.org
--------------------------------------------------------------------
 
 
 
Subject:
[Spm] blunt pyramidal tips
From:
<valeria.cassina at unimib.it>
Date:
Fri, 25 Jul 2008 17:42:28 +0200
To:
spm at spmlist.di.com

 

To:

spm at spmlist.di.com


Dear Colleagues,
I'm looking for blunt pyramidal tips for cell elasticity measurements,
can you suggest me where to buy it?

thank you
Valeria
____________________________________________

Dott. Valeria Cassina

Dipartimento di Medicina Sperimentale
Facolta' di Medicina e Chirurgia
Universita' di Milano - Bicocca
Via Cadore 48
20052 Monza (Milano)
Italy

Tel:                    +39-02-6448 8243
Fax:                     +39-02-6448 8068
E-mail:          valeria.cassina at unimib.it
____________________________________________


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