[Spm] Re: Spm Digest, Vol 53, Issue 1 - different deflection sensitivity in two phases (Sam Engler)

[Olivier Arnould]

Hello,

Is it not "simply" due to stiffness change between phases? I expect 
something like that and you'll only need to change you cantilever to adapt 
its stiffness to those of your phases if necessary.

Best regards,

Olivier Arnould

> Message: 4
> Date: Sun, 30 Nov 2008 20:28:44 +0000
> From: Sam Engler <smtygtyg at hotmail.com>
> Subject: [Spm] different deflection sensitivity in two phases
> To: <spm at spmlist.di.com>
> Message-ID: <BAY119-W10DE244018FB1F05C3000AD1060 at phx.gbl>
> Content-Type: text/plain; charset="iso-8859-1"
>
>
>
> Hello Everyone,
>
> I have a multiple composition lipid membrane sample, which phase-separated 
> into 3 phases. During the force-displacement measurements, the slopes of 
> the f-d curves within phase C are different than the slopes within phase A 
> and B. I've tried different cantilevers and this is consistent. My 
> questions are (1) why the deflection sensitivity of the cantilever is 
> changing from one phase to another phase? (2) should I change to stiffer 
> cantilevers?
>
> Any comments will be appreciated.
>
> Thanks,
> Sam,
>
> Sam Engler, Ph.D