[Spm] Cleaning waffle calibration gratings (Don Chernoff at ASM)
dipak paramanik
dipak1205 at yahoo.co.in
Tue Aug 21 11:03:00 EDT 2007
Dear Don,
From my five years experience of working with AFM, I have understood that the
best way to clean the grating (or other surface) is just by blowinng argon gas on the surface. During blowing the gas, you should control the gas pressure so that it will not hurt the sample surafce.
You can try this and let me know the result.
Cheers !
Dipak
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Today's Topics:
1. Re: consult about grain size analysis (dg liu) (dipak paramanik)
2. Cleaning waffle calibration gratings (Don Chernoff at ASM)
3. spherical probe (Michal Wozniak)
4. membrane for mechanical fixation living cells (Michal Wozniak)
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Message: 1
Date: Sat, 18 Aug 2007 15:54:29 +0100 (BST)
From: dipak paramanik
Subject: [Spm] Re: consult about grain size analysis (dg liu)
To: spm at spmlist.di.com
Message-ID: <373617.26043.qm at web8612.mail.in.yahoo.com>
Content-Type: text/plain; charset="iso-8859-1"
Dear Liu,
You can only see the number of paritcles having the a size by clicking the mouse on the histogram plot. There is no option to export the full hisogram plot in to a ascii file. However you can export the file into ascii file which will show you the all the particle sizes present in your picture, you choose the unit in nm while exporting.
Then you export this ascii file into origin. In origin you can plot as histogram.
You can try this and let me know if you have any problem.
regards
Dipak Paramanik
Doctoral Scholar
Institute of Physics, India
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Message: 1
Date: Sat, 18 Aug 2007 03:25:22 +0800
From: "dg liu"
Subject: [Spm] consult about grain size analysis
To: spm at spmlist.di.com
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Dear all,
I am writing to consult you about a technical question on "AFM grain size
analysis".
I am now using DI (now Veeco) multimode Nano IIIa for AFM experiment.
I am trying to compare the grain size distribution of different samples.
While doing grain size analysis, I intend to export the hist% figure (for
AFM manu when doing grain size analysis, it shows the hist% ) into ASCII
file, so that I can edit and compare the grain size distribution of
different samples by Excel.
Whether it is possible to expert the hist% figure into ASCII file? if it is
possible, how can I do it?
Your professional opinion is highly appreciated.
Thank you very much.
Dage Liu
Research associate
Univeristy of Wisconsin-Madison
CNT center
phone: 919-208-8582(mobile)
email: dliu5 at wisc.edu
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Message: 2
Date: Mon, 20 Aug 2007 12:11:59 -0400
From: "Don Chernoff at ASM"
Subject: [Spm] Cleaning waffle calibration gratings
To: "SPM List"
Message-ID: <003101c7e344$d9d143c0$6701a8c0 at asm15>
Content-Type: text/plain; charset="iso-8859-1"
Many AFMs are supplied with a 'waffle grating' to calibrate the XYZ axes. Typically, the pattern consists of 5 um wide pits on a square grid with 10 um pitch. The pits are normally 180 nm deep (older gratings) or 200 nm deep (newer gratings). Many of the older gratings seem to accumulate small bumps (< 0.5 um wide) that are 10-50 nm high.
What cleaning techniques are useful for these gratings so that nano-dirt does not interfere with the measurements? For example, has anyone tried CO2 snow cleaning? Plasma cleaning?
I myself have tried ultrasonic cleaning, using an ordinary small lab unit (not megasonic). This succeeded in breaking large clumps into smaller ones and spreading them across the surface, so that the specimen was less useful than before.
regards,
Don
=============================================
Don Chernoff, Ph.D., President
Advanced Surface Microscopy, Inc. E-Mail: donc at asmicro.com
3250 N. Post Rd., Ste. 120 Voice: 317-895-5630
INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada)
web: http://www.asmicro.com Fax: 317-895-5652
[business activities: analytical services in AFM, AFM probes, consulting, training,
calibration and test specimens, calibration and measurement software,
used NanoScope equipment.]
=============================================
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Message: 3
Date: Mon, 20 Aug 2007 14:45:51 +0900
From: "Michal Wozniak"
Subject: [Spm] spherical probe
To: spm at spmlist.di.com
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<22019d2c0708192245v64058e30s41205611881f787d at mail.gmail.com>
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Dear SPMlist Users
I would like to know who can offer me spherical shape unmountaed probe
with low spring constant for the force measurements on a single living
cells.
Thank you in advance for your help
Michal Wozniak
--
Michal J. Wozniak Ph.D.
Organoid Group
Biomaterials Center
National Institute for Materials Science
1-1 Namiki, Tsukuba, Ibaraki 3050044
Japan
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Message: 4
Date: Mon, 20 Aug 2007 17:35:53 +0900
From: "Michal Wozniak"
Subject: [Spm] membrane for mechanical fixation living cells
To: spm at spmlist.di.com
Message-ID:
<22019d2c0708200135l249fb5carf721541fff1f5f8c at mail.gmail.com>
Content-Type: text/plain; charset="iso-8859-1"
Dear SPMlist Users
I would like to know who can offer me a membrane for mechanical fixation
living cells. I am interested in membranes with a holes size from about 30
up to 150 um.
Thank you in advance for your help
Michal Wozniak
--
Michal J. WOZNIAK Ph.D.
Organoid Group
Biomaterials Center
National Institute for Materials Science
1-1 Namiki, Tsukuba, Ibaraki 3050044
Japan
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